Compendium Of In Vivo Monitoring In Real-time Molecular Neuroscience - Volume 2: Microdialysis And Sensing Of Neural Tissues

Compendium Of In Vivo Monitoring In Real-time Molecular Neuroscience - Volume 2: Microdialysis And Sensing Of Neural Tissues

Volume 2: Microdialysis and Sensing of Neural Tissues

George S Wilson, Adrian C Michael;;;


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Volume 1 of this series focused mainly on small molecules which are intrinsically electroactive. Volume 2 continues to provide current approaches to real time measurements of essential species in the central nervous system. It describes microdialysis, a sampling technique, which facilitates the removal of small volumes of solution containing the analyte(s) of interest which can be subsequently separated by chromatography and analyzed by a range of techniques. These include electrochemistry, UV-Vis spectroscopy, fluorescence and mass spectroscopy. This approach affords the opportunity to measure a range of targets in real-time thus providing access to pharmacokinetic/pharmacodynamics measurements affording the possibility of measuring the disappearance of a drug after administration and the subsequent appearance of metabolites. Microdialysis further affords the ability to sample peptides and proteins of neurobiological interest.

Consistent with the goals of Volume 1, Volume 2 seeks to provide for scientists in the field of neurobiology a base of bioanalytical techniques that may not be familiar to them. Specific examples are provided and the fundamental basis of the technique is also covered.

  • Separation-Based Methods Combined with Microdialysis Sampling for Monitoring Neurotransmitters and Drug Delivery to the Brain (Rachel A Saylor, Sara R Thomas and Susan M Lunte)
  • Microdialysis for Pharmacokinetic and Pharmacodynamic Studies with Focus on the CNS (Margareta Hammarlund-Udenaes)
  • Real-Time Neurochemical Monitoring Using Microdialysis (Michelle L Rogers and Martyn G Boutelle)
  • In Vivo Brain Microdialysis in Human Subjects During Cognitive Tests (Robert J Buchanan, Conor M Gerace and Klevest Gjini)
  • Advanced Microdialysis Approaches Resolve Differences in Serotonin Homeostasis and Signaling (Maureen M Sampson, Hongyan Yang and Anne M Andrews)
  • Improving Temporal Resolution in Online Microdialysis/Liquid Chromatography (Jing Zhang, Khanh T Ngo, Stephen R Groskreutz and Stephen G Weber)
  • Liquid Chromatography-Mass Spectrometry Analysis of Dialysate and Applications with Selective Neuronal Stimulation (Jenny-Marie T Wong, Samuel S Pappas and Robert T Kennedy)
  • Derivatization Chemistries for Improved Detection of Monoamine Neurotransmitters and Their Metabolites in Microdialysis Samples by Liquid Chromatography with Fluorescence Detection and Tandem Mass Spectrometry (Jan Kehr and Takashi Yoshitake)
  • Microdialysis Contributions to the Understanding of Reward and Addiction (Roy A Wise and Zhi-Bing You)
  • Voltammetry Reveals How Mesolimbic Dopamine Relates to Instrumental Reward Seeking (Kate M Wassum)
  • In Vivo Neurochemical Monitoring of the Crab Cancer Borealis via Microdialysis and Mass Spctrometry (Zhidan Liang and Lingiun Li)
  • In Vivo Microdialysis Coupled with Selective Online Electrochemical Systems to Study Ascorbate Dynamics in the Rat Brain (Meining Zhang, Fei Wu and Langqun Mao)
  • Open Volume Microfluidic Probes (Gavin D M Jeffries, Anna A Kim, Kiryl Kustanovich and Aldo Jesorka)
  • Push–Pull Perfusion Sampling of Thin Neuronal Tissues (Scott A Shippy and Geovannie Ojeda-Torres)
  • Microdialysis Flux Considerations (Julie A Stenken and Samuel L Patton)
  • Advancing Intracranial Microdialysis Sampling and Measurements (Andrea Jaquins-Gerstl, Erika L Varner, Kathryn M Nesbitt and Adrian C Michael)

Readership: Students and researchers in biochemistry, neurobiology, neuroanatomy, biomedical sciences and bioengineering.
Microdialysis;Microfluidics;Sensors;Neurotransmitters;Pharmacokinetics;Pharmacodynamics;Traumatic Brain Injury;UHPLC;LC-MSKey Features:
  • Excellent balance between the development and validation of methodology combined with investigators focused on detailed understanding of neurobiological processes